Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation

نویسندگان

  • Kui Chen
  • Mingyi Zhang
  • Ya-Nan Chang
  • Lin Xia
  • Weihong Gu
  • Yanxia Qin
  • Juan Li
  • Suxia Cui
  • Gengmei Xing
چکیده

The surface plasmon resonance (SPR) effect endows gold nanoparticles (GNPs) with the ability to visualize biomolecules. In the present study, we designed and constructed a GNP probe to allow the semi-quantitative analysis of methylated tumor suppressor genes in cultured cells. To construct the probe, the GNP surfaces were coated with single-stranded DNA (ssDNA) by forming Au-S bonds. The ssDNA contains a thiolated 5'-end, a regulatory domain of 12 adenine nucleotides, and a functional domain with absolute pairing with methylated p16 sequence (Met-p16). The probe, paired with Met-p16, clearly changed the color of aggregating GNPs probe in 5 mol/L NaCl solution. Utilizing the probe, p16 gene methylation in HCT116 cells was semi-quantified. Further, the methylation of E-cadherin, p15, and p16 gene in Caco2, HepG2, and HCT116 cell lines were detected by the corresponding probes, constructed with three domains. This simple and cost-effective method was useful for the diagnosis of DNA methylation-related diseases.

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عنوان ژورنال:

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2016